human smad1 shrna (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
human smad1 shrna
Human Smad1 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+smad1+shrna/Smad1+shRNA+(h)+Lentiviral+Particles/pmc06048458-97-2-15
Average 93 stars, based on 4 article reviews
Human Smad1 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+smad1+shrna/Smad1+shRNA+(h)+Lentiviral+Particles/pmc06048458-97-2-15
Average 93 stars, based on 4 article reviews
human smad1 shrna - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Overproduction of growth differentiation factor 15 promotes human rhinovirus infection and virus-induced inflammation in the lung"
Article Title: Overproduction of growth differentiation factor 15 promotes human rhinovirus infection and virus-induced inflammation in the lung
Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology
doi: 10.1152/ajplung.00324.2017
Figure Legend Snippet: Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) defines genome-wide transcriptional regulation by pSmad1 protein upon growth differentiation factor 15 (GDF15) in human airway epithelial cells. Normal human tracheobronchial epithelial cells in submerged culture were treated with 0.1% BSA-HCl (control) or recombinant human GDF15 (25 ng/ml) for 2 h to perform the phosphorylated-Smad1 (pSmad1) ChIP-seq analysis.
Techniques Used: Chromatin Immunoprecipitation, Next-Generation Sequencing, ChIP-sequencing, Genome Wide, Control, Recombinant
![Overexpressing human growth differentiation factor 15 (GDF15) enhances activation of Smad1 and interferon regulatory factor 7 (IRF7) in mouse airway epithelial cells. Primary tracheal epithelial cells from naïve human GDF15 transgenic (hGDF15 Tg+) mice or wild-type (WT) littermates were grown at air-liquid interface and infected with HRV-1B [multiplicity of infection (MOI) 1] or PBS (control) for 24 h. A, left: quantitative Western blot densitometry data of phosphorylated-Smad1 (pSmad1) and total Smad1 protein. A, right: representative Western blot pictures; B, left: quantitative Western blot densitometry data of IRF7 and β-actin protein (loading control). B, right: representative Western blot pictures. Data are presented as means ± SE from three independent experiments. *P < 0.05, **P < 0.01, compared with PBS controls or HRV-infected WT cells.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8458/pmc06048458/pmc06048458__zh50021873860008.jpg "... growth differentiation factor 15 (GDF15) enhances activation of Smad1 and interferon regulatory factor 7 (IRF7) in mouse ...")
Figure Legend Snippet: Overexpressing human growth differentiation factor 15 (GDF15) enhances activation of Smad1 and interferon regulatory factor 7 (IRF7) in mouse airway epithelial cells. Primary tracheal epithelial cells from naïve human GDF15 transgenic (hGDF15 Tg+) mice or wild-type (WT) littermates were grown at air-liquid interface and infected with HRV-1B [multiplicity of infection (MOI) 1] or PBS (control) for 24 h. A, left: quantitative Western blot densitometry data of phosphorylated-Smad1 (pSmad1) and total Smad1 protein. A, right: representative Western blot pictures; B, left: quantitative Western blot densitometry data of IRF7 and β-actin protein (loading control). B, right: representative Western blot pictures. Data are presented as means ± SE from three independent experiments. *P < 0.05, **P < 0.01, compared with PBS controls or HRV-infected WT cells.
Techniques Used: Activation Assay, Transgenic Assay, Infection, Control, Western Blot
![Smad1 cooperates with interferon regulatory factor 7 (IRF7) in response to HRV infection via growth differentiation factor 15 (GDF15) signaling in human airway epithelial cells. A: normal human bronchial epithelial cells transduced with lentiviruses encoding human GDF15 shRNA (shGDF15) or control shRNA (shControl) were grown at air-liquid interface and infected with HRV-16 [multiplicity of infection (MOI) 1] or PBS (control) for 24 h. A, top: quantitative Western blot densitometry data of IRF7 and β-actin protein (loading control). A, bottom: representative Western blot pictures. B, top: normal human tracheobronchial epithelial cells transduced with lentiviruses encoding human Smad1 shRNA (shSmad1) or control shRNA (shControl) were grown at air-liquid interface for 28 days to examine Smad1 protein expression. B, bottom: quantitative Western blot densitometry data of Smad1 and GAPDH protein (loading control). C: normal human tracheobronchial epithelial cells transduced with lentiviruses encoding human Smad1 shRNA (shSmad1) or control shRNA (shControl) were grown at air-liquid interface, pretreated with 0.1% BSA-HCl (control) or recombinant human GDF15 (25 ng/ml) for 72 h, and infected with HRV-16 (MOI 1) or PBS for 24 h. C, top: quantitative Western blot densitometry data of IRF7 and β-actin protein (loading control). C, bottom: representative Western blot pictures. Data are presented as means ± SE from 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared with PBS controls or shControl cells; NS, not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8458/pmc06048458/pmc06048458__zh50021873860009.jpg "Smad1 cooperates with interferon regulatory factor 7 (IRF7) in ...")
Figure Legend Snippet: Smad1 cooperates with interferon regulatory factor 7 (IRF7) in response to HRV infection via growth differentiation factor 15 (GDF15) signaling in human airway epithelial cells. A: normal human bronchial epithelial cells transduced with lentiviruses encoding human GDF15 shRNA (shGDF15) or control shRNA (shControl) were grown at air-liquid interface and infected with HRV-16 [multiplicity of infection (MOI) 1] or PBS (control) for 24 h. A, top: quantitative Western blot densitometry data of IRF7 and β-actin protein (loading control). A, bottom: representative Western blot pictures. B, top: normal human tracheobronchial epithelial cells transduced with lentiviruses encoding human Smad1 shRNA (shSmad1) or control shRNA (shControl) were grown at air-liquid interface for 28 days to examine Smad1 protein expression. B, bottom: quantitative Western blot densitometry data of Smad1 and GAPDH protein (loading control). C: normal human tracheobronchial epithelial cells transduced with lentiviruses encoding human Smad1 shRNA (shSmad1) or control shRNA (shControl) were grown at air-liquid interface, pretreated with 0.1% BSA-HCl (control) or recombinant human GDF15 (25 ng/ml) for 72 h, and infected with HRV-16 (MOI 1) or PBS for 24 h. C, top: quantitative Western blot densitometry data of IRF7 and β-actin protein (loading control). C, bottom: representative Western blot pictures. Data are presented as means ± SE from 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared with PBS controls or shControl cells; NS, not significant.
Techniques Used: Infection, Transduction, shRNA, Control, Western Blot, Expressing, Recombinant
Related Articles
shRNA:Article Title: Cigarette Smoke Induces Human Airway Epithelial Senescence via Growth Differentiation Factor 15 Production Article Snippet: Grant Support: This study was supported by The Flight Attendant Medical and Research Institute (FAMRI) Young Clinical Scientist Award (grant number 123254) to QW & FAMRI Clinical Innovator Award (grant number 130041) to HWC.. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.. Page 1 of 47 AJRCMB Articles in Press. Article Title: Overproduction of growth differentiation factor 15 promotes human rhinovirus infection and virus-induced inflammation in the lung Article Snippet: The MISSION lentiviral vector containing human GDF15 shRNA (TRCN000005839, pLKO.1-shRNA-hGDF15, shGDF15) or control shRNA (SHC001, shControl) was purchased from Sigma-Aldrich, and lentiviruses were generated as previously described ( 52 ). .. Lentiviruses encoding Control:Article Title: Cigarette Smoke Induces Human Airway Epithelial Senescence via Growth Differentiation Factor 15 Production Article Snippet: Grant Support: This study was supported by The Flight Attendant Medical and Research Institute (FAMRI) Young Clinical Scientist Award (grant number 123254) to QW & FAMRI Clinical Innovator Award (grant number 130041) to HWC.. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.. Page 1 of 47 AJRCMB Articles in Press. Article Title: Overproduction of growth differentiation factor 15 promotes human rhinovirus infection and virus-induced inflammation in the lung Article Snippet: The MISSION lentiviral vector containing human GDF15 shRNA (TRCN000005839, pLKO.1-shRNA-hGDF15, shGDF15) or control shRNA (SHC001, shControl) was purchased from Sigma-Aldrich, and lentiviruses were generated as previously described ( 52 ). .. Lentiviruses encoding |
![Knockdown of growth differentiation factor 15 (GDF15) reduces viral load and enhances IFN-λ1/IL-29 expression during HRV-16 infection in human airway epithelial cells. Brushed normal human bronchial epithelial cells transduced with lentiviruses encoding human GDF15 shRNA (shGDF15) or control shRNA (shControl) were grown at air-liquid interface and infected with HRV-16 [multiplicity of infection (MOI) 1] or PBS (control) for 24 h. A: HRV RNA levels were measured by quantitative real-time RT-PCR. B: released virus particles in apical supernatants (ASN) were assessed by plaque assay. C: IFN-λ1/IL-29 mRNA levels were measured by quantitative real-time RT-PCR. D: IFN-λ1/IL-29 protein levels in ASN were assessed by ELISA. Relative levels of mRNA or RNA expression were normalized to GAPDH mRNA levels and calculated via the ΔΔCt method. Data are presented as means ± SE from 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared with PBS controls or shControl cells; ND, Not detectable.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8458/pmc06048458/pmc06048458__zh50021873860005.jpg)
![Knockdown of growth differentiation factor 15 (GDF15) reduces virus-induced inflammation during HRV-16 infection in human airway epithelial cells. Normal human bronchial epithelial cells transduced with lentiviruses encoding human GDF15 shRNA (shGDF15) or control shRNA (shControl) were grown at air-liquid interface and infected with HRV-16 [multiplicity of infection (MOI) 1] or PBS (control) for 24 h. Protein levels of IP-10 (A), IL-6 (B), IL-8 (C), and GDF15 (D) in apical supernatants (ASN) were assessed by ELISA. Data are presented as means ± SE from four independent experiments. *P < 0.05, ***P < 0.001, compared with PBS controls or shControl cells; NS, not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8458/pmc06048458/pmc06048458__zh50021873860006.jpg)